pLIB-TSP-UL32
(Plasmid
#250566)
-
PurposeInsect cell expression of HSV-1 UL32 with an N-terminal TwinStrep tag and HRV 3C protease cleavage site
-
Depositing Lab
-
Depositing OrganizationYale University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250566 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepLIB
-
Vector typeInsect Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin and Gentamicin, 100 & 10 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameUL32
-
SpeciesHSV-1 (HHV-1)
- Promoter polyhedrin
-
Tag
/ Fusion Protein
- TwinStrepTagII-HRV3C (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLIB-TSP-UL32 was a gift from Allison Didychuk (Addgene plasmid # 250566 ; http://n2t.net/addgene:250566 ; RRID:Addgene_250566) -
For your References section:
Conserved assembly architecture of the essential herpesvirus packaging accessory factor. Bailey EJ, Devarkar SC, Szczepaniak R, Meissner LM, Chen X, Wu C, Weller SK, Xiong Y, Didychuk AL. PLoS Pathog. 2026 Sep 10;22(9):e1014540. doi: 10.1371/journal.ppat.1014540. eCollection 2026 Sep. 10.1371/journal.ppat.1014540 PubMed 42721160