Conserved assembly architecture of the essential herpesvirus packaging accessory factor.
Bailey EJ, Devarkar SC, Szczepaniak R, Meissner LM, Chen X, Wu C, Weller SK, Xiong Y, Didychuk AL
PLoS Pathog. 2026 Sep 10;22(9):e1014540. doi: 10.1371/journal.ppat.1014540. eCollection 2026 Sep.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 250565 | pLIB-TSP-ORF68 | Insect cell expression of KSHV ORF68 with an N-terminal TwinStrep tag and HRV 3C protease cleavage site |
| 250566 | pLIB-TSP-UL32 | Insect cell expression of HSV-1 UL32 with an N-terminal TwinStrep tag and HRV 3C protease cleavage site |
| 250567 | pLIB-TSP-UL52 | Insect cell expression of HCMV UL52 with an N-terminal TwinStrep tag and HRV 3C protease cleavage site |
| 250568 | pcDNA4/TO-2xStrep-UL32 12-596 | Expression of N-terminal 2x-Strep-tagged HSV-1 UL32 residues 12-596 in mammalian cells |
| 250569 | pcDNA4/TO-2xStrep-UL32 C297A | Expression of N-terminal 2x-Strep-tagged HSV-1 UL32 C297A in mammalian cells |
| 250570 | pcDNA4/TO-2xStrep-UL32 C308A | Expression of N-terminal 2x-Strep-tagged HSV-1 UL32 C308A in mammalian cells |
| 250571 | pcDNA4/TO-2xStrep-UL32 C309A | Expression of N-terminal 2x-Strep-tagged HSV-1 UL32 C309A in mammalian cells |
| 250572 | pcDNA4/TO-2xStrep-UL32 K289A/R293A/R301A | Expression of N-terminal 2x-Strep-tagged HSV-1 UL32 K289A/R293A/R301A in mammalian cells |
| 250573 | pcDNA4/TO-2xStrep-UL32 K532A/C535A | Expression of N-terminal 2x-Strep-tagged HSV-1 UL32 K532A/C535A in mammalian cells |
| 250574 | pcDNA4/TO-2xStrep-UL32 K563A | Expresses N-terminal 2x-Strep-tagged HSV-1 UL32 K563A in mammalian cells |
| 250575 | pcDNA4/TO-2xStrep-UL32 R366A/R370A | Expresses N-terminal 2x-Strep-tagged HSV-1 UL32 R366A/R370A in mammalian cells |
| 250576 | pcDNA4/TO-2xStrep-UL32 R440E | Expresses N-terminal 2x-Strep-tagged HSV-1 UL32 R440E in mammalian cells |
| 250577 | pcDNA4/TO-2xStrep-UL32 R572A | Expresses N-terminal 2x-Strep-tagged HSV-1 UL32 R572A in mammalian cells |
| 250578 | pcDNA4/TO-2xStrep-UL32 R579A/R580A | Expresses N-terminal 2x-Strep-tagged HSV-1 UL32 R579A/R580A in mammalian cells |