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pcDNA4/TO-2xStrep-UL32 R572A
(Plasmid #250577)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250577 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA4/TO
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UL32
  • Species
    HSV-1 (HHV-1)
  • Mutation
    Amino acid R572 mutated to alanine
  • Promoter CMV
  • Tag / Fusion Protein
    • TwinStrepTagII (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4/TO-2xStrep-UL32 R572A was a gift from Allison Didychuk (Addgene plasmid # 250577 ; http://n2t.net/addgene:250577 ; RRID:Addgene_250577)

  • For your References section:

    Conserved assembly architecture of the essential herpesvirus packaging accessory factor. Bailey EJ, Devarkar SC, Szczepaniak R, Meissner LM, Chen X, Wu C, Weller SK, Xiong Y, Didychuk AL. PLoS Pathog. 2026 Sep 10;22(9):e1014540. doi: 10.1371/journal.ppat.1014540. eCollection 2026 Sep. 10.1371/journal.ppat.1014540 PubMed 42721160
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