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SpyTag-mGreenLantern-A AND C-SpyTag
(Plasmid #250632)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250632 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET 29b (+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MGSS-CfaC-GGS(x3)-SpyTag-GGS(x3)-2A9c-GGS(x3)-mGreenLantern-GGS(x3)-SpyTag-GGS(x3)- HRV-3Cc-GGS(x3)-6xHis-GGS(x3)-CfaN-SsrA
  • Species
    Synthetic
  • Tag / Fusion Protein
    • 6x His tag, SsrA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SpyTag-mGreenLantern-A AND C-SpyTag was a gift from Cole DeForest (Addgene plasmid # 250632 ; http://n2t.net/addgene:250632 ; RRID:Addgene_250632)

  • For your References section:

    Boolean Logic-Based Control Over Recombinant Protein Biomaterial Degradability and Therapeutic Delivery. Ross ML, Gharios R, Kottantharayil S, DeForest CA. Adv Mater. 2026 Jul 21:e74038. doi: 10.1002/adma.74038. 10.1002/adma.74038 PubMed 42478326
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