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Boolean Logic-Based Control Over Recombinant Protein Biomaterial Degradability and Therapeutic Delivery.

Ross ML, Gharios R, Kottantharayil S, DeForest CA
Adv Mater. 2026 Jul 21:e74038. doi: 10.1002/adma.74038. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
250626SpyTag-mGreenLantern-A-SpyTagExpresses crosslink enabling YES-gated degradation and release of mGreenLantern from materials in response to eSrtA(2A9) treatment. Construct contains two SpyTags for material crosslinking.
250627SpyTag-mGreenLantern-C-SpyTagExpresses crosslink enabling YES-gated degradation and release of mGreenLantern from materials in response to HRV-3C treatment. Construct contains two SpyTags for material crosslinking.
250628SpyTag-mGreenLantern-T-SpyTagExpresses crosslink enabling YES-gated degradation and release of mGreenLantern from materials in response to TEV treatment. Construct contains two SpyTags for material crosslinking.
250629SpyTag-mGreenLantern-A OR C-SpyTagExpresses crosslink enabling OR-gated degradation and release of mGreenLantern from materials in response to eSrtA(2A9) OR HRV-3C treatment. Construct contains two SpyTags for material crosslinking.
250630SpyTag-mGreenLantern-A OR T-SpyTagExpresses crosslink enabling OR-gated degradation and release of mGreenLantern from materials in response to eSrtA(2A9) OR TEV treatment. Construct contains two SpyTags for material crosslinking.
250631SpyTag-mGreenLantern-T OR C-SpyTagExpresses crosslink enabling OR-gated degradation and release of mGreenLantern from materials in response to TEV OR HRV-3C treatment. Construct contains two SpyTags for material crosslinking.
250632SpyTag-mGreenLantern-A AND C-SpyTagExpresses crosslink enabling AND-gated degradation and release of mGreenLantern from materials in response to eSrtA(2A9) AND HRV-3C treatment. Construct contains two SpyTags for material crosslinking.
250633SpyTag-mGreenLantern-A AND T-SpyTagExpresses crosslink enabling AND-gated degradation and release of mGreenLantern from materials in response to eSrtA(2A9) AND TEV treatment. Construct contains two SpyTags for material crosslinking.
250634SpyTag-mGreenLantern-T AND C-SpyTagExpresses crosslink enabling AND-gated degradation and release of mGreenLantern from materials in response to TEV AND HRV-3C treatment. Construct contains two SpyTags for material crosslinking.
250635SpyTag-mGreenLantern-(A OR C) AND T-SpyTagExpresses crosslink enabling (OR)AND-gated degradation and release of mGL from materials in response to [eSrtA(2A9) OR HRV-3C] AND TEV treatment. Construct contains two SpyTags for material creation.
250636SpyTag-mGreenLantern-(A OR T) AND C-SpyTagExpresses crosslink enabling (OR)AND-gated degradation and release of mGL from materials in response to [eSrtA(2A9) OR TEV] AND HRV-3C treatment. Construct contains two SpyTags for material creation.
250637SpyTag-mGreenLantern-(T OR C) AND A-SpyTagExpresses crosslink enabling (OR)AND-gated degradation and release of mGL from materials in response to (TEV OR HRV-3C) AND eSrtA(2A9) treatment. Construct contains two SpyTags for material creation.
250638SpyTag-mGreenLantern-(A AND C) OR T-SpyTagExpresses crosslink enabling (AND)OR-gated degradation and release of mGL from materials in response to [eSrtA(2A9) AND HRV-3C] OR TEV treatment. Construct contains two SpyTags for material creation.
250639SpyTag-mGreenLantern-(A AND T) OR C-SpyTagExpresses crosslink enabling (AND)OR-gated degradation and release of mGL from materials in response to [eSrtA(2A9) AND TEV] OR HRV-3C treatment. Construct contains two SpyTags for material creation.
250640SpyTag-mGreenLantern-(T AND C) OR A-SpyTagExpresses crosslink enabling (AND)OR-gated degradation and release of mGL from materials in response to (TEV AND HRV-3C) OR eSrtA(2A9) treatment. Construct contains two SpyTags for material creation.
250641SpyTag-mGreenLantern-A OR T OR C-SpyTagExpresses crosslink enabling OROR-gated degradation and release of mGL from materials in response to eSrtA(2A9) OR TEV OR HRV-3C treatment. Construct contains two SpyTags for material creation.
250642SpyTag-mGreenLantern-A AND C AND T-SpyTagExpresses crosslink enabling ANDAND-gated degradation and release of mGL from materials in response to eSrtA(2A9) AND HRV-3C AND TEV treatment. Construct contains two SpyTags for material creation.
250643ELP-TriSpyCatcherExpresses ELP material polymer with three SpyCatchers, enabling material creation via SpyLigation when mixed with crosslinks that contain at least two SpyTags.
250644SpyTag-EGF-A AND C-SpyTagExpresses crosslink enabling AND-gated degradation and release of EGF from materials in response to eSrtA(2A9) AND HRV-3C treatment. Construct contains two SpyTags for material crosslinking.
250645SpyTag-MBP-A AND C-SpyTagExpresses crosslink enabling AND-gated degradation and release of MBP from materials in response to eSrtA(2A9) AND HRV-3C treatment. Construct contains two SpyTags for material crosslinking.
250646SpyTag-EGF-(A AND T) OR C-SpyTagExpresses crosslink enabling (AND)OR-gated degradation and release of EGF from materials in response to [eSrtA(2A9) AND TEV] OR HRV-3C treatment. Construct contains two SpyTags for material creation.
250647SpyTag-MBP-(A AND T) OR C-SpyTagExpresses crosslink enabling (AND)OR-gated degradation and release of MBP from materials in response to [eSrtA(2A9) AND TEV] OR HRV-3C treatment. Construct contains two SpyTags for material creation.
250648SpyTag-MBP-A OR T OR C-SpyTagExpresses crosslink enabling OROR-gated degradation and release of MBP from materials in response to eSrtA(2A9) OR TEV OR HRV-3C treatment. Construct contains two SpyTags for material crosslinking.
250649emiRFP703Encodes for cytosolic mammalian expression of a near-infrared fluorescent protein (emiRFP703) that excites at 703 nm.

Antibodies from Article