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pcDNA3.1-endo-GαsBP2 WT-mKate2
(Plasmid #250685)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250685 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    endo-G_sBP2 WT-mKate2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1311
  • Mutation
    none
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-endo-GαsBP2 WT-mKate2 was a gift from Mikel Garcia-Marcos (Addgene plasmid # 250685 ; http://n2t.net/addgene:250685 ; RRID:Addgene_250685)

  • For your References section:

    Inhibitory probes for spatiotemporal analysis of Galpha(s) protein signaling. Zhao J, Luebbers A, Savransky S, Lin TY, Cheng N, Wilcox A, Janicot R, Green E, Sharma A, Maziarz M, Varelas X, Irannejad R, Vilardaga JP, Garcia-Marcos M. Nat Chem Biol. 2026 Feb 3. doi: 10.1038/s41589-025-02138-1. 10.1038/s41589-025-02138-1 PubMed 41634441
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