Inhibitory probes for spatiotemporal analysis of Galpha(s) protein signaling.
Zhao J, Luebbers A, Savransky S, Lin TY, Cheng N, Wilcox A, Janicot R, Green E, Sharma A, Maziarz M, Varelas X, Irannejad R, Vilardaga JP, Garcia-Marcos M
Nat Chem Biol. 2026 Feb 3. doi: 10.1038/s41589-025-02138-1.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 250683 | pcDNA3.1-mem-GαsBP2 WT-mKate2 | Expressing plasma membrane anchored GαsBP2 WT |
| 250684 | pcDNA3.1-mem-GαsBP2 WA-mKate2 | Expressing plasma membrane anchored GαsBP2 W11A inactive mutant |
| 250685 | pcDNA3.1-endo-GαsBP2 WT-mKate2 | Expressing endosome located GαsBP2 WT |
| 250686 | pcDNA3.1-endo-GαsBP2 WA-mKate2 | Expressing endosome located GαsBP2 W11A inactive mutant |
| 250687 | pcDNA3.1-cyto-GαsBP2 WT-mKate2 | Expressing cytosol located GαsBP2 WT |
| 250688 | pcDNA3.1-cyto-GαsBP2 WA-mKate2 | Expressing cytosol located GαsBP2 W11A inactive mutant |
| 250689 | pmRFP-FKBP-GαsBP2 | Expressing FKBP fused GαsBP2 WT |
| 250690 | pmCherry-N1-Lyn11-FRB | Expressing plasma membrane achored FRB |
| 250691 | pmRFP-PYLcs-GαsBP2 | Expressing PYLcs fused GαsBP2 WT |
| 250692 | pcDNA3.1-Lyn11-ABIcs | Expressing plasma membrane achored ABIcs |
| 250693 | pLVX-CMV-GαsBP2 WT-mKate2-mem-IRES-Hygro | Expressing plasma membrane anchored GαsBP2 WT |
| 250694 | pLVX-CMV-GαsBP2 WA-mKate2-mem-IRES-Hygro | Expressing plasma membrane anchored GαsBP2 W11A inactive mutant |
| 250695 | pLVX-CMV-mem-GαsBP2 WT-mKate2-IRES-Hygro | Expressing plasma membrane anchored GαsBP2 WT |
| 250696 | pLVX-CMV-mem-GαsBP2 WA-mKate2-IRES-Hygro | Expressing plasma membrane anchored GαsBP2 W11A inactive mutant |
| 250697 | pLVX-CMV-endo-GαsBP2 WT-mKate2-IRES-Hygro | Expressing endosome located GαsBP2 WT |
| 250698 | pLVX-CMV-endo-GαsBP2 WA-mKate2-IRES-Hygro | Expressing endosome located GαsBP2 W11A inactive mutant |
| 250699 | pLVX-CMV-cyto-GαsBP2 WT-mKate2-IRES-Hygro | Expressing cytosol located GαsBP2 WT |
| 250700 | pLVX-CMV-cyto-GαsBP2 WA-mKate2-IRES-Hygro | Expressing cytosol located GαsBP2 W11A inactive mutant |
| 250701 | pcDNA3.1-Gαz-YFP | Expressing Citrine version of YFP fused human Gαz WT |
| 250702 | pcDNA3.1-Gαz Q205L-YFP | Expressing Citrine version of YFP fused human Gαz Q205L |
| 250703 | pcDNA3.1-mas-Nluc | Expressing plasma membrane anchored nanoluciferase (Nluc) |
| 250704 | pcDNA3.1-mas-GαsBP2-Nluc | Expressing plasma membrane anchored GαsBP2 WT fused to nanoluciferase (Nluc) |
| 250705 | pcDNA3.1-mas-GαsBP1-Nluc | Expressing plasma membrane anchored GαsBP1 WT fused to nanoluciferase (Nluc) |
| 250706 | pmRFP-FKBP-Ric8A | Expressing FKBP fused Ric8A |
| 250707 | pcDNA3.1-Gαs(long) | Expressing human Gαs WT, long isoform |
| 250708 | pcDNA3.1-Gαs R201C (short) | Expressing human Gαs R201C mutant, short isoform |
| 250709 | pcDNA3.1-Gαs Q227L (short) | Expressing human Gαs Q227L mutant, short isoform |
| 250710 | pcDNA3.1-Gαs S275L (short) | Expressing human Gαs S275L mutant, short isoform |
| 250711 | pcDNA3.1-Gαs 99-Venus (short) | Expressing Venus funsed human Gαs R201C, short isoform |
| 250712 | pcDNA3.1-Gαs 99-Venus R201C (short) | Expressing Venus funsed human G_s R201C, short isoform |
| 250713 | pcDNA3.1-Gαs 99-Venus Q227L (short) | Expressing Venus funsed human G_s Q227L, short isoform |
| 250714 | pGEX-KG-GαsBP1 | Expressing GST fused GαsBP1 |
| 250715 | pGEX-KG-GαsBP2 | Expressing GST fused GαsBP2 |