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pmRFP-PYLcs-GαsBP2
(Plasmid #250691)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250691 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pmRFP-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PYLcs-G_sBP2 WT
  • Species
    H. sapiens (human)
  • Mutation
    none
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unspecified (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmRFP-PYLcs-GαsBP2 was a gift from Mikel Garcia-Marcos (Addgene plasmid # 250691 ; http://n2t.net/addgene:250691 ; RRID:Addgene_250691)

  • For your References section:

    Inhibitory probes for spatiotemporal analysis of Galpha(s) protein signaling. Zhao J, Luebbers A, Savransky S, Lin TY, Cheng N, Wilcox A, Janicot R, Green E, Sharma A, Maziarz M, Varelas X, Irannejad R, Vilardaga JP, Garcia-Marcos M. Nat Chem Biol. 2026 Feb 3. doi: 10.1038/s41589-025-02138-1. 10.1038/s41589-025-02138-1 PubMed 41634441
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