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pmCherry-N1-Lyn11-FRB
(Plasmid #250690)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250690 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pmCherry1
  • Modifications to backbone
    The original backbone of this plasmid is pmCherry, and an ECFP was inserted. Then, the mCherry was removed, and a stop codon was inserted before the ECFP sequence.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lyn11-FRB
  • Species
    H. sapiens (human)
  • Mutation
    none
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherry-N1-Lyn11-FRB was a gift from Mikel Garcia-Marcos (Addgene plasmid # 250690 ; http://n2t.net/addgene:250690 ; RRID:Addgene_250690)

  • For your References section:

    Inhibitory probes for spatiotemporal analysis of Galpha(s) protein signaling. Zhao J, Luebbers A, Savransky S, Lin TY, Cheng N, Wilcox A, Janicot R, Green E, Sharma A, Maziarz M, Varelas X, Irannejad R, Vilardaga JP, Garcia-Marcos M. Nat Chem Biol. 2026 Feb 3. doi: 10.1038/s41589-025-02138-1. 10.1038/s41589-025-02138-1 PubMed 41634441
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