pLVX-CMV-mem-GαsBP2 WA-mKate2-IRES-Hygro
(Plasmid
#250696)
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PurposeExpressing plasma membrane anchored GαsBP2 W11A inactive mutant
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250696 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLVX-IRES-Hygro
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemem-G_sBP2 W11A-mKate2
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SpeciesH. sapiens (human)
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MutationW11A in the G_sBP2 sequence
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX-CMV-mem-GαsBP2 WA-mKate2-IRES-Hygro was a gift from Mikel Garcia-Marcos (Addgene plasmid # 250696 ; http://n2t.net/addgene:250696 ; RRID:Addgene_250696) -
For your References section:
Inhibitory probes for spatiotemporal analysis of Galpha(s) protein signaling. Zhao J, Luebbers A, Savransky S, Lin TY, Cheng N, Wilcox A, Janicot R, Green E, Sharma A, Maziarz M, Varelas X, Irannejad R, Vilardaga JP, Garcia-Marcos M. Nat Chem Biol. 2026 Feb 3. doi: 10.1038/s41589-025-02138-1. 10.1038/s41589-025-02138-1 PubMed 41634441