dpYPQ230-RRV-intron-ABE
(Plasmid
#250718)
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PurposedLbCas12a-RRV-intron based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-RRV-intron with the linker of (GGGGS)x8
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250718 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepYPQ167
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameecTadA8e-(GGGGS)x8-dLbCas12a-RRV-intron
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Tag
/ Fusion Protein
- 5' and 3' NLS (N terminal on backbone)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
dpYPQ230-RRV-intron-ABE was a gift from Yiping Qi (Addgene plasmid # 250718 ; http://n2t.net/addgene:250718 ; RRID:Addgene_250718) -
For your References section:
Enhancing CRISPR-Cas12a base editing in plants with LbCas12a variants and introns. Cheng Y, Li G, Zhou M, Mandlik R, Wang D, Qi Y. J Integr Plant Biol. 2026 Apr 2. doi: 10.1111/jipb.70249. 10.1111/jipb.70249 PubMed 41928060