Enhancing CRISPR-Cas12a base editing in plants with LbCas12a variants and introns.
Cheng Y, Li G, Zhou M, Mandlik R, Wang D, Qi Y
J Integr Plant Biol. 2026 Apr 2. doi: 10.1111/jipb.70249.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 250716 | dpYPQ230-RRV-ABE | dLbCas12a-RRV based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-RRV with the linker of (GGGGS)x8 |
| 250718 | dpYPQ230-RRV-intron-ABE | dLbCas12a-RRV-intron based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-RRV-intron with the linker of (GGGGS)x8 |
| 250719 | dpYPQ230-RRV-intron-CBE | dLbCas12a-RRV-intron based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-RRV-intron with the linker of (GGGGS)x6 |
| 250923 | pYPQ142-AtUBQ10 | Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs driven by AtUBQ10 promoter |
| 250924 | pYPQ144-AtUBQ10-pT | Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs driven by AtUBQ10 promoter |
| 250985 | pYPQ143-AtUBQ10 | Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs driven by AtUBQ10 promoter |