pAPIC-soma-AcrIIA4
(Plasmid
#250802)
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PurposeDrosophila transgenic vector: To make tub-AcrIIA4 with piRNA targeting sequence in the 3'UTR; to suppress Cas9 activity in somatic tissues but not in the germline
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250802 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepAPIC
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAcrIIA4
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SpeciesD. melanogaster (fly)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains AcrIIA4. Contains D10A. Please visit https://doi.org/10.64898/2025.12.07.692848 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAPIC-soma-AcrIIA4 was a gift from Chun Han (Addgene plasmid # 250802 ; http://n2t.net/addgene:250802 ; RRID:Addgene_250802) -
For your References section:
Harnessing anti-CRISPR to suppress, subtract, and segregate Cas9 activity for precision CRISPR in Drosophila. Shen Y, Sheen M, Yeung AT, Chen X, Wang B, Huang Z, Ho CA, Lakkis ZI, Han C. bioRxiv 2025.12.07.692848 10.64898/2025.12.07.692848