Harnessing anti-CRISPR to suppress, subtract, and segregate Cas9 activity for precision CRISPR in Drosophila
Shen Y, Sheen M, Yeung AT, Chen X, Wang B, Huang Z, Ho CA, Lakkis ZI, Han C
bioRxiv 2025.12.07.692848
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 250800 | pDEST-APIC-AcrIIA4 | Drosophila transgenic vector: Gateway cloning destination vector for expressing enhancer driven AcrIIA4 |
| 250801 | pAPIC-nos-AcrIIA4 | Drosophila transgenic vector: To express AcrIIA4 driven by nos transcription regulatory sequences (promoter, 5'UTR, 3'UTR and polyA); to suppress Cas9 activity in the germline |
| 250802 | pAPIC-soma-AcrIIA4 | Drosophila transgenic vector: To make tub-AcrIIA4 with piRNA targeting sequence in the 3'UTR; to suppress Cas9 activity in somatic tissues but not in the germline |