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pdTomato-TD
(Plasmid #250944)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250944 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pRSETb
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2887
  • Total vector size (bp) 4423
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dTomato Tandem Dimer
  • Species
    Synthetic
  • Insert Size (bp)
    1503
  • Promoter T7
  • Tag / Fusion Protein
    • 6His (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminator
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pdTomato-TD was a gift from Steven Vogel (Addgene plasmid # 250944 ; http://n2t.net/addgene:250944 ; RRID:Addgene_250944)

  • For your References section:

    Anomalous photophysical behaviors attributed to excitonic coupling in fluorescent proteins. Nguyen T, Puhl HL, Chen E, Hines K, Rani C, Blank PS, Carlotti B, Kim Y, Goodson T 3rd, Vogel SS. Biophys J. 2025 Dec 2;124(23):4293-4309. doi: 10.1016/j.bpj.2025.10.022. Epub 2025 Oct 18. 10.1016/j.bpj.2025.10.022 PubMed 41109955
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