Anomalous photophysical behaviors attributed to excitonic coupling in fluorescent proteins.

Nguyen T, Puhl HL, Chen E, Hines K, Rani C, Blank PS, Carlotti B, Kim Y, Goodson T 3rd, Vogel SS
Biophys J. 2025 Dec 2;124(23):4293-4309. doi: 10.1016/j.bpj.2025.10.022. Epub 2025 Oct 18. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
250938	pdVenus-TD	Expresses a 6-His tagged tandem dimer of the fluorescent protein dVenus in bacteria.
250939	pdVenus-TDX	Expresses a 6-His tagged tandem dimer of the fluorescent protein dVenus in bacteria. The second dVenus lacks a chromophore.
250940	pdLanYFP-TD	Expresses a 6-His tagged tandem dimer of the fluorescent protein dLanYFP in bacteria.
250942	pdLanYFP-TDX	Expresses a 6-His tagged tandem dimer of the fluorescent protein dLanYFP in bacteria. The second dLanYFP lacks a chromophore.
250944	pdTomato-TD	Expresses a 6-His tagged tandem dimer of the fluorescent protein dTomato in bacteria.
250945	pdTomato-TDX	Expresses a 6-His tagged tandem dimer of the fluorescent protein dTomato in bacteria. The second dTomato lacks a chromophore.
250946	p4nmTriangle	Expresses a putative triangle with dTomato-mVenus-mVenusHP-dTomato in mammalian cells. Each fluorescent protein is separated by a 4nm ER/K helix.
250947	p20nmTriangle	Expresses a putative triangle with dTomato-mVenus-mVenusHP-dTomato in mammalian cells. Each fluorescent protein is separated by a 20nm ER/K helix.
250948	p60nmTriangle	Expresses a putative triangle with dTomato-mVenus-mVenusHP-dTomato in mammalian cells. Each fluorescent protein is separated by a 60nm ER/K helix.