p60nmTriangle
(Plasmid
#250948)
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PurposeExpresses a putative triangle with dTomato-mVenus-mVenusHP-dTomato in mammalian cells. Each fluorescent protein is separated by a 60nm ER/K helix.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250948 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 11974
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name60nm Triangle
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SpeciesSynthetic
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Insert Size (bp)6639
- Promoter CMV
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Tag
/ Fusion Protein
- 6His (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMVfor
- 3′ sequencing primer BGHrev
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p60nmTriangle was a gift from Steven Vogel (Addgene plasmid # 250948 ; http://n2t.net/addgene:250948 ; RRID:Addgene_250948) -
For your References section:
Anomalous photophysical behaviors attributed to excitonic coupling in fluorescent proteins. Nguyen T, Puhl HL, Chen E, Hines K, Rani C, Blank PS, Carlotti B, Kim Y, Goodson T 3rd, Vogel SS. Biophys J. 2025 Dec 2;124(23):4293-4309. doi: 10.1016/j.bpj.2025.10.022. Epub 2025 Oct 18. 10.1016/j.bpj.2025.10.022 PubMed 41109955