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p30T crRNA55/120
(Plasmid #250980)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250980 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pHERD30T
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Selection in Pseudomonas aeruginosa requires ~50 µg/mL of gentamicin. ~15 µg/mL is sufficient in E. coli.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dual CRISPR array expressing two Cas13a crRNAs to target orf55 and orf120 in phiKZ

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.11.23.690004 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p30T crRNA55/120 was a gift from Joseph Bondy-Denomy (Addgene plasmid # 250980 ; http://n2t.net/addgene:250980 ; RRID:Addgene_250980)

  • For your References section:

    Bacteriophage genome-wide transposon mutagenesis. Chan A, Yee WX, Mozumdar D, Kokontis C, Rojas-Montero M, Liu M, Yang Y, Yuping L, Bondy-Denomy J. Cell. 2026 Jul 23;189(15):4810-4824.e7. doi: 10.1016/j.cell.2026.06.030. 10.1016/j.cell.2026.06.030 PubMed 42492495
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