Bacteriophage genome-wide transposon mutagenesis.
Chan A, Yee WX, Mozumdar D, Kokontis C, Rojas-Montero M, Liu M, Yang Y, Yuping L, Bondy-Denomy J
Cell. 2026 Jul 23;189(15):4810-4824.e7. doi: 10.1016/j.cell.2026.06.030.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 250980 | p30T crRNA55/120 | Expresses two Cas13a guide RNAs targeting orf55 and orf120 in phiKZ |
| 250981 | pUCminiTn7-Mariner-sfC-nlp1 | pUC mini-Tn7 inserting Mariner transposase-sfCherry-nlp1 into genome |
| 250982 | pUCminiTn7-Mariner-flag | pUC mini-Tn7 inserting Mariner transposase-flag into genome |
| 250983 | pHERD30T-TnV1acrVIA1 | pHERD30T vector providing the transposon donor with acrVIA1 |
| 250984 | pHERD20T-V3TnAcrVIA1 | pHERD20T vector providing the transposon donor with acrVIA1 |
| 260819 | pHERD20T-V3Tn(anti-END) | pHERD20T vector providing the transposon donor with anti-END |