pMVP-blast-mCherry-LGALS8
(Plasmid
#251003)
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PurposeLentiviral vector with constitutive expression of mCherry tagged human galectin 8
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251003 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepMVP/Lenti/Blast-DEST
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Backbone manufacturerChristopher Newgard (Addgene plasmid #121849)
- Backbone size w/o insert (bp) 9188
- Total vector size (bp) 10142
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namegalectin 8
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Alt nameLGALS8
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SpeciesH. sapiens (human)
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Insert Size (bp)951
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GenBank IDAH008815
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Entrez GeneLGALS8 (a.k.a. Gal-8, PCTA-1, PCTA1, Po66-CBP)
- Promoter EF-1a
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Tag
/ Fusion Protein
- mCherry (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMVP-blast-mCherry-LGALS8 was a gift from Jörn Coers (Addgene plasmid # 251003 ; http://n2t.net/addgene:251003 ; RRID:Addgene_251003)