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Toggle Switch - LuxI
(Plasmid #251147)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251147 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pColE1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    LacI
  • Promoter PLtetO-1

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    sfGFP-LuxI
  • Promoter PLtetO-1

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    TetR
  • Promoter trc, lacO

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    mCerulean
  • Promoter trc, lacO

Cloning Information for Gene/Insert 4

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Toggle Switch - LuxI was a gift from Yolanda Schaerli (Addgene plasmid # 251147 ; http://n2t.net/addgene:251147 ; RRID:Addgene_251147)

  • For your References section:

    An engineered multi-step differentiation program in Escherichia coli for self-organized spatial patterning. Boni E, Siboulet H, Ceracchini G, Aor I, Holló G, Kusumawardhani H, Schaerli Y. eLife15:RP110624 10.7554/eLife.110624.1
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