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An engineered multi-step differentiation program in Escherichia coli for self-organized spatial patterning

Boni E, Siboulet H, Ceracchini G, Aor I, Holló G, Kusumawardhani H, Schaerli Y
eLife15:RP110624 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
251146Toggle SwitchToggle Switch (TetR - mCerulean / LacI - GFP)
251147Toggle Switch - LuxIToggle Switch (TetR - mCerulean / LacI - GFP - LuxI)
251148C6 deviceContains LuxR receptor and expresses mCherry in response to 3O-C6-HSL
251149C14 deviceContains CinR receptor and expresses eYFP in response to 3O-C14-HSL
251150C6_C14_deviceContains LuxR receptor and expresses mCherry and CinI synthase in response to 3O-C6-HSL. Contains CinR receptor and expresses mCitrine in response to 3O-C14-HSL.

Antibodies from Article