C6_C14_device
(Plasmid #251150)

• Purpose: Contains LuxR receptor and expresses mCherry and CinI synthase in response to 3O-C6-HSL. Contains CinR receptor and expresses mCitrine in response to 3O-C14-HSL.
• Depositing Lab: Yolanda Schaerli
• Publication: Boni et al eLife15:RP110624

Backbone

• Vector backbone: pCDF
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: mCherry
• Promoter: RBS

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: LuxR
• Promoter: trc, lacO

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 3

• Gene/Insert name: CinI
• Promoter: pLuxLac

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 4

• Gene/Insert name: mCitrine
• Promoter: cinO

Cloning Information for Gene/Insert 4

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 5

• Gene/Insert name: CinR
• Promoter: trc, lacO

Cloning Information for Gene/Insert 5

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry