C6 device
(Plasmid
#251148)
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PurposeContains LuxR receptor and expresses mCherry in response to 3O-C6-HSL
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251148 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCDF
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Modifications to backboneLuxR is not preceded by any obvious RBS, but is expressed at sufficiently high levels to drive mCherry expression in response to 3O-C6-HSL.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namemCherry
- Promoter pLux76
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameLuxR
- Promoter trc, lacO
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
C6 device was a gift from Yolanda Schaerli (Addgene plasmid # 251148 ; http://n2t.net/addgene:251148 ; RRID:Addgene_251148) -
For your References section:
An engineered multi-step differentiation program in Escherichia coli for self-organized spatial patterning. Boni E, Siboulet H, Ceracchini G, Aor I, Holló G, Kusumawardhani H, Schaerli Y. eLife15:RP110624 10.7554/eLife.110624.1
Map uploaded by the depositor.
