pHAGE-pEF1a-FBXO7(1-522)_pGK-BlastR
(Plasmid
#251191)
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PurposeLentiviral expression in Mammalian cells of untagged FBXO7 and selection with Blasticidin S
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251191 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepHAGE- pEF1alpha, pGK BlastR
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Backbone manufacturerStephen Elledge Lab
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFBXO7
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SpeciesH. sapiens (human)
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Entrez GeneFBXO7 (a.k.a. FBX, FBX07, FBX7, PARK15, PKPS)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAGE-pEF1a-FBXO7(1-522)_pGK-BlastR was a gift from Wade Harper (Addgene plasmid # 251191 ; http://n2t.net/addgene:251191 ; RRID:Addgene_251191) -
For your References section:
Regulation of the proteasome 20S core particle by the Parkinsonism-associated Proteins FBXO7 and PI31. Adolf F, Goodall EA, Singh E, von Gronau S, Fermin Perez E, Fung D, Müller B, Paulo JA, Hanna J, Harper JW, Schulman BA. bioRxiv 2026.05.28.728263 10.64898/2026.05.28.728263