Regulation of the proteasome 20S core particle by the Parkinsonism-associated Proteins FBXO7 and PI31
Adolf F, Goodall EA, Singh E, von Gronau S, Fermin Perez E, Fung D, Müller B, Paulo JA, Hanna J, Harper JW, Schulman BA
bioRxiv 2026.05.28.728263
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 251177 | pHAGE-N-FLAG-HA-FBXO7(1-522)-IRES-PuroR | Lentiviral expression in Mammalian cells of N-terminally FLAG and HA-tagged FBXO7 and selection with Puromycin |
| 251178 | pHAGE-N-FLAG-HA-FBXO7(1-406)-IRES-PuroR | Lentiviral expression in Mammalian cells of N-terminally FLAG and HA-tagged FBXO7 truncation 1-406 and selection with Puromycin |
| 251188 | pHAGE-N-FLAG-HA-FBXO7(169-522)-IRES-PuroR | Lentiviral expression in Mammalian cells of N-terminally FLAG and HA-tagged FBXO7 containing PD mutation R498X, a truncation encoding residues 1-497 and selection with Puromycin |
| 251189 | pHAGE-N-FLAG-HA-FBXO7(169-406)-IRES-PuroR | Lentiviral expression in Mammalian cells of N-terminally FLAG and HA-tagged FBXO7 containing PD mutation R498X, a truncation encoding residues 1-497 and selection with Puromycin |
| 251191 | pHAGE-pEF1a-FBXO7(1-522)_pGK-BlastR | Lentiviral expression in Mammalian cells of untagged FBXO7 and selection with Blasticidin S |
| 251192 | pHAGE-pEF1a-PSMF1(1-271)_pGK-BlastR | Lentiviral expression in Mammalian cells of untagged PSMF1 and selection with Blasticidin S |
| 251193 | pHAGE-pEF1a-PSMF1(1-151)_pGK-BlastR | Lentiviral expression in Mammalian cells of untagged PSMF1 truncation, residues 1-151 and selection with Blasticidin S |
| 251194 | pLEX305-pGK-N-dTAG-HA-PSMF1(1-271)_PuroR | Lentiviral expression in Mammalian cells of N-terminally dTAG and HA-tagged PSMF1 and selection with Puromycin |
| 251195 | pLEX305-pGK-N-dTAG-HA-PSMF1(1-151)_PuroR | Lentiviral expression in Mammalian cells of N-terminally dTAG and HA-tagged PSMF1 truncation, resideus 1-151 and selection with Puromycin |
| 251196 | pLEX305-pGK-N-dTAG-HA-PSMF1(L53M)_PuroR | Lentiviral expression in Mammalian cells of N-terminally dTAG and HA-tagged PSMF1 containing L53M point mutation and selection with Puromycin |
| 251197 | pLEX305-pGK-N-dTAG-HA-PSMF1(L228V)_PuroR | Lentiviral expression in Mammalian cells of N-terminally dTAG and HA-tagged PSMF1 containing L228V point mutation and selection with Puromycin |
| 251198 | pLEX305-pGK-N-dTAG-HA-PSMF1(1-230_R231X)_PuroR | Lentiviral expression in Mammalian cells of N-terminally dTAG and HA-tagged PSMF1 containing point mutation R231X leading to the truncation encoding residues 1-230 and selection with Puromycin |
| 251199 | pLEX305-pGK-N-dTAG-HA-PSMF1(R242G)_PuroR | Lentiviral expression in Mammalian cells of N-terminally dTAG and HA-tagged PSMF1 containing R242G point mutation and selection with Puromycin |
| 251200 | pLEX305-pGK-N-dTAG-HA-PSMF1(R242C)_PuroR | Lentiviral expression in Mammalian cells of N-terminally dTAG and HA-tagged PSMF1 containing R242C point mutation and selection with Puromycin |
| 251201 | pDONR223 FBXO7(1-522) | Gateway-compatible pDONR vector containing FBXO7 (1-522) and a stop codon |
| 251202 | pDONR223 PSMF1(1-271) | Gateway-compatible pDONR vector containing PSMF1 (1-271) and a stop codon |
| 251203 | pX458- PSMF1 guide 1 | Transient transfection-compatible vector driving expression of spCas9 and production of sgRNA targeting exon2 of PSMF1 |
| 251204 | pX458- PSMF1 guide 2 | Transient transfection-compatible vector driving expression of spCas9 and production of sgRNA targeting exon2 of PSMF1 |