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pLEX305-pGK-N-dTAG-HA-PSMF1(L53M)_PuroR
(Plasmid #251196)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251196 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLEX_305-N-dTAG
  • Backbone manufacturer
    James Bradner and Behnam Nabet, addgene #91797
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PSMF1
  • Alt name
    PI31
  • Species
    H. sapiens (human)
  • Mutation
    L53M
  • Entrez Gene
    PSMF1 (a.k.a. PI31)
  • Tag / Fusion Protein
    • dTAG(FKBP12F36V)-HA (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEX305-pGK-N-dTAG-HA-PSMF1(L53M)_PuroR was a gift from Wade Harper (Addgene plasmid # 251196 ; http://n2t.net/addgene:251196 ; RRID:Addgene_251196)

  • For your References section:

    Regulation of the proteasome 20S core particle by the Parkinsonism-associated Proteins FBXO7 and PI31. Adolf F, Goodall EA, Singh E, von Gronau S, Fermin Perez E, Fung D, Müller B, Paulo JA, Hanna J, Harper JW, Schulman BA. bioRxiv 2026.05.28.728263 10.64898/2026.05.28.728263
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