DENV_1ED3
(Plasmid
#251246)
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PurposeTo express the dengue virus serotype 1 envelope protein domain III in E. coli
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251246 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepET-15b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDENV_1ED3
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SpeciesDengue virus serotype 1
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Tag
/ Fusion Protein
- 6xHis, 11 aa linker, thrombin cleavage site (N terminal on backbone)
Cloning Information
- Cloning method Gene Synthesis
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DENV_1ED3 was a gift from Yutaka Kuroda (Addgene plasmid # 251246 ; http://n2t.net/addgene:251246 ; RRID:Addgene_251246) -
For your References section:
The Immunogenicity of DENV1-4 ED3s Strongly Differ despite Their Almost Identical Three-Dimensional Structures and High Sequence Similarities. Islam MD, Sharmin T, Tipo IH, Saha A, Yesmin S, Roy MG, Brindha S, Kuroda Y, Islam MM. Int J Mol Sci. 2023 Jan 25;24(3):2393. doi: 10.3390/ijms24032393. 10.3390/ijms24032393 PubMed 36768719