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DENV_4ED3
(Plasmid #251249)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251249 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET-15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DENV_4ED3
  • Species
    Dengue virus serotype 4
  • Tag / Fusion Protein
    • 6xHis, 11 aa linker, thrombin cleavage site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DENV_4ED3 was a gift from Yutaka Kuroda (Addgene plasmid # 251249 ; http://n2t.net/addgene:251249 ; RRID:Addgene_251249)

  • For your References section:

    Computational prediction and experimental characterization of a "size switch type repacking" during the evolution of dengue envelope protein domain III (ED3). Elahi M, Islam MM, Noguchi K, Yohda M, Toh H, Kuroda Y. Biochim Biophys Acta. 2014 Mar;1844(3):585-92. doi: 10.1016/j.bbapap.2013.12.013. Epub 2013 Dec 27. 10.1016/j.bbapap.2013.12.013 PubMed 24373879
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