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Corona_Omic_RBD
(Plasmid #251258)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251258 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET-15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Corona_Omic_RBD
  • Species
    SARS-CoV-2 Omicron variant
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Tag / Fusion Protein
    • 6xHis, 11 aa linker, thrombin cleavage site (N terminal on backbone)

Cloning Information

  • Cloning method Other

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Corona_Omic_RBD was a gift from Yutaka Kuroda (Addgene plasmid # 251258 ; http://n2t.net/addgene:251258 ; RRID:Addgene_251258)

  • For your References section:

    E. coli production of a multi-disulfide bonded SARS-CoV-2 Omicron BA.5 RBD exhibiting native-like biochemical and biophysical properties. Wongnak R, Brindha S, Yoshizue T, Onchaiya S, Mizutani K, Kuroda Y. Biophys Physicobiol. 2023 Sep 21;20(4):e200036. doi: 10.2142/biophysico.bppb-v20.0036. eCollection 2023. 10.2142/biophysico.bppb-v20.0036 PubMed 38344033
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