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frq[ΔNLS, mNG_V5]_nat-1
(Plasmid #251399)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251399 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRS426
  • Total vector size (bp) 13236
  • Vector type
    Neurospora Expression
  • Selectable markers
    Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    frq
  • Alt name
    NCU02265
  • Species
    Neurospora crassa
  • Mutation
    deleted amino acids 95-100
  • GenBank ID
    XM_011396823
  • Tags / Fusion Proteins
    • mNeonGreen (C terminal on insert)
    • V5 (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    frq[ΔNLS, mNG_V5]_nat-1 was a gift from Jay C Dunlap (Addgene plasmid # 251399 ; http://n2t.net/addgene:251399 ; RRID:Addgene_251399)

  • For your References section:

    Rhythmic Nuclear Import Mediated by Importins Regulates the Neurospora Circadian Clock. Wang Z, Wang B, Bartholomai BM, Loros JJ, Dunlap JC. Genetics. 2026 May 18:iyag126. doi: 10.1093/genetics/iyag126. 10.1093/genetics/iyag126 PubMed 42144878
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