Rhythmic Nuclear Import Mediated by Importins Regulates the Neurospora Circadian Clock.

Wang Z, Wang B, Bartholomai BM, Loros JJ, Dunlap JC
Genetics. 2026 May 18:iyag126. doi: 10.1093/genetics/iyag126. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
251399	frq[ΔNLS, mNG_V5]_nat-1	Encodes an NLS1-deleted frq allele with C-terminal mNeonGreen and V5 tags, enabling fluorescent visualization and detection following replacement of the endogenous frq locus.
251400	nup159[mApple]_bar	Enables endogenous C-terminal tagging of the nuclear pore complex component NCU08604, which encodes the homolog of yeast Nup159, with mApple.
251401	nup211[mTagBFP2]_hph	Enables endogenous C-terminal tagging of the nuclear pore complex component NCU04059, which encodes the homolog of yeast Mlp1/2, with mTagBFP2.
251402	csr-1_qa-2_dsimpα_bar	Enables inducible expression of double-stranded RNA targeting importin α (NCU01249) under the control of the qa-2 promoter, with the expression cassette inserted at the csr-1 locus.
251403	wc-1[ΔLOV, mAp]_bar	Encodes an LOV-deleted wc-1 allele with C-terminal mApple tag, enabling fluorescent visualization and detection following replacement of the endogenous wc-1 locus.
251404	qa-2_[VHF]impβ3_bar	Enables qa-2 promoter-driven inducible expression and endogenous N-terminal VHF tagging of NCU02357, encoding importin β3. The insert contains the bar resistance gene as a drug selection marker.
251405	qa-2_[VHF]impβ3_nat-1	Enables qa-2 promoter-driven inducible expression and endogenous N-terminal VHF tagging of NCU02357, encoding importin β3, and includes the nourseothricin resistance gene for drug selection.