qa-2_[VHF]impβ3_bar
(Plasmid
#251404)
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PurposeEnables qa-2 promoter-driven inducible expression and endogenous N-terminal VHF tagging of NCU02357, encoding importin β3. The insert contains the bar resistance gene as a drug selection marker.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251404 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepRS426
- Total vector size (bp) 9690
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Vector typeNeurospora Expression
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Selectable markersphosphinothricin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameimportin beta 3
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Alt nameNCU02357
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SpeciesNeurospora crassa
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Tags
/ Fusion Proteins
- V5 (N terminal on insert)
- 10xHis (N terminal on insert)
- 3xFlag (N terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
qa-2_[VHF]impβ3_bar was a gift from Jay C Dunlap (Addgene plasmid # 251404 ; http://n2t.net/addgene:251404 ; RRID:Addgene_251404) -
For your References section:
Rhythmic Nuclear Import Mediated by Importins Regulates the Neurospora Circadian Clock. Wang Z, Wang B, Bartholomai BM, Loros JJ, Dunlap JC. Genetics. 2026 May 18:iyag126. doi: 10.1093/genetics/iyag126. 10.1093/genetics/iyag126 PubMed 42144878
Map uploaded by the depositor.
