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pJHCi
(Plasmid #251729)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251729 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCAH01SpR
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.05.28.656465 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJHCi was a gift from Calvin Henard (Addgene plasmid # 251729 ; http://n2t.net/addgene:251729 ; RRID:Addgene_251729)

  • For your References section:

    CRISPRi-based functional genomic screening identifies genes essential for CH(4)-dependent growth in a methanotrophic bacterium. Henard JM, Lee SA, Yu YC, Shao D, Azad RK, Henard CA. Sci Adv. 2026 Apr 24;12(17):eaed4234. doi: 10.1126/sciadv.aed4234. Epub 2026 Apr 24. 10.1126/sciadv.aed4234 PubMed 42030398
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