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CRISPRi-based functional genomic screening identifies genes essential for CH(4)-dependent growth in a methanotrophic bacterium.

Henard JM, Lee SA, Yu YC, Shao D, Azad RK, Henard CA
Sci Adv. 2026 Apr 24;12(17):eaed4234. doi: 10.1126/sciadv.aed4234. Epub 2026 Apr 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
251729pJHCibroad-host-range plasmid for CRISPR interference; inducible dCas9; sgRNA expression from methanotroph constitutive promoter

Antibodies from Article