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Addgene

NEDD5
(Plasmid #25184)

Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 25184 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pET28a-LIC
  • Backbone manufacturer
    SGC
  • Backbone size w/o insert (bp) 7328
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NEDD5:E13-Q320-B3_W260A
  • Alt name
    PDB: 2QNR
  • Alt name
    Septin 2, GTPase domain
  • Alt name
    NEDD5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    924
  • Mutation
    Contains amino acids E13-Q320. W260A mutation.
  • Entrez Gene
    SEPTIN2 (a.k.a. DIFF6, NEDD-5, NEDD5, Pnutl3, SEPT2, hNedd5)
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NEDD5 was a gift from Cheryl Arrowsmith (Addgene plasmid # 25184 ; http://n2t.net/addgene:25184 ; RRID:Addgene_25184)