Bxb1-GT_AIO-TetOn_Divergent_Donor_KanR
(Plasmid
#252232)
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PurposeBxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible (trans)gene expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252232 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBR322/pUC
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Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLacZ; rtTA-T2A-mTagBFP2
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SpeciesSynthetic
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Insert Size (bp)6500
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MutationN/A
- Promoter hEF1a; TRE; CAG
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Bxb1-GT_AIO-TetOn_Divergent_Donor_KanR was a gift from Kate Galloway (Addgene plasmid # 252232 ; http://n2t.net/addgene:252232 ; RRID:Addgene_252232)
Included in kit:
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