Kate Galloway Lab: Engineering STRAIGHT-IN single and dual lines in the male iPS11 parental line for programmable DNA integration
Blanch-Asensio A, Gumustop DR, Wang NB, Peterman EL, Ploessl DS, Galloway KE
Stem Cell Research, 2026. In submission.
These materials are included in the STRAIGHT-IN Dual Kit.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 252223 | CLYBL_Bxb1-GT_CAG-EBFP_LP _TC | CLYBL targeting construct with the Bxb1-GT landing pad cassette expressing EBFP |
| 252224 | CLYBL_Bxb1-GA_CAG-EGFP_LP _TC | CLYBL targeting construct with the Bxb1-GA landing pad cassette expressing EGFP |
| 252225 | CLYBL_Bxb1-GA_CAG-mSc-ALFA_LP _TC | CLYBL targeting construct with the Bxb1-GA landing pad cassette expressing mScarlet tagged with ALFA tag |
| 252226 | CLYBL_CAG-TALEN-L1 | TALEN-expressing construct to target CLYBL (left) |
| 252227 | CLYBL_CAG-TALEN-R1 | TALEN-expressing construct to target CLYBL (right) |
| 252228 | Bxb1-GT-Donor_CBh_mCherry | Bxb1-GT donor plasmid for cloning DNA payloads upon NotI digest |
| 252229 | Bxb1-GA-Donor_CBh_mCherry | Bxb1-GA donor plasmid for cloning DNA payloads upon NotI digest |
| 252230 | Bxb1-GT-CAG_Donor_KanR | Bxb1-GT donor plasmid for cloning (trans)genes upon NotI digest, expressed under CAG promoter and upstream WPRE+polyA |
| 252231 | Bxb1-GA-CAG_Donor_KanR | Bxb1-GA donor plasmid for cloning (trans)genes upon NotI digest, expressed under CAG promoter and upstream WPRE+polyA |
| 252232 | Bxb1-GT_AIO-TetOn_Divergent_Donor_KanR | Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible (trans)gene expression |
| 252233 | Bxb1-GA_AIO-TetOn_Divergent_Donor_KanR | Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible (trans)gene expression |
| 252234 | Bxb1-GT-CAG-mGL | Bxb1-GT donor plasmid for constitutive mGreenLantern expression |
| 252235 | Bxb1-GA-CAG-iRFP670 | Bxb1-GA donor plasmid for constitutive iRFP670 expression |
| 252236 | Bxb1-GT-Dox-ETV2_Div | Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible ETV2 expression |
| 252237 | Bxb1-GA-Dox-NKX3-1_Div | Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible NKX3.1 expression |
| 252238 | CAG-eeBxb1_bGH | eeBxb1-expressing plasmid under CAG promoter |
| 252239 | pIVT-desNb_EGFP-PuroR-mRuby2 | Template for IVTR of destabilized antibody against EGFP or EBFP with BleoR-mRuby2 as effector |
| 252240 | pIVT-desNb_EGFP-BleoR-mRuby2 | Template for IVTR of destabilized antibody against EGFP or EBFP with PuroR-mRuby2 as effector |
| 252241 | pIVT-wtNb_ALFA-PuroR-mRuby2 | Template for IVTR of wt antibody against ALFA tag with PuroR-mRuby2 as effector |