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pcDNA3.1-miRFP670nano3-mRuby2
(Plasmid #252343)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252343 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Thermo Fisher (Invitrogen)
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miRFP670nano3-mRuby2
  • Species
    Synthetic
  • Insert Size (bp)
    1212
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV_F
  • 3′ sequencing primer BGH_R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Restriction cloned from pcDNA3.1-Clover-mRuby2 (#49089) by inserting miRFP670nano3 using a synthetic gene block with KpnI/BamHI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-miRFP670nano3-mRuby2 was a gift from James Conway (Addgene plasmid # 252343 ; http://n2t.net/addgene:252343 ; RRID:Addgene_252343)

  • For your References section:

    Red-to-Near-Infrared Fluorescence Resonance Energy Transfer Reporters for Dual Imaging Applications. Tran VT, Ek F, Yassine H, Behera R, Hytonen VP, Conway JRW. Small Methods. 2026 Jul 12:e70828. doi: 10.1002/smtd.70828. 10.1002/smtd.70828 PubMed 42438328
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