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pOPINB-yItt1 full-length WT (1-464)
(Plasmid #252506)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252506 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pOPINB
  • Backbone size w/o insert (bp) 5313
  • Total vector size (bp) 6735
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E3 ubiquitin-protein ligase ITT1
  • Alt name
    Inhibitor of Translation Termination 1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1395
  • GenBank ID
    NM_001182427
  • Promoter T7

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOPINB-yItt1 full-length WT (1-464) was a gift from Bernhard Lechtenberg (Addgene plasmid # 252506 ; http://n2t.net/addgene:252506 ; RRID:Addgene_252506)

  • For your References section:

    The C-terminal tail of the RBR E3 ligase RNF14defines branched K6/K48 polyubiquitin specificity in ribosomal quality control. Trevelyan SJ, Riedel J, Tong YH, Dong H, Jiou J, McGowan ENS, Wang XS, Cobbold SA, Feltham R, Lechtenberg BC. 2026 Molecular Cell, 86. 10.1016/j.molcel.2026.10.024
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