The C-terminal tail of the RBR E3 ligase RNF14defines branched K6/K48 polyubiquitin specificity in ribosomal quality control
Trevelyan SJ, Riedel J, Tong YH, Dong H, Jiou J, McGowan ENS, Wang XS, Cobbold SA, Feltham R, Lechtenberg BC
2026 Molecular Cell, 86.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 252502 | pOPINB-hRNF14 full-length WT(1-474) | Expression of human RNF14 full-length wild-type protein, codon optimized for E. coli |
| 252503 | pOPINB-hRNF14 full-length C417A (1-474) | Expression of human RNF14 full-length active site mutant (C417A) protein, codon optimized for E. coli |
| 252504 | pOPINB-hRNF14 RBR WT (178-474) | Expression of human RNF14 RBR domain wild-type protein, codon optimized for E. coli |
| 252505 | pOPINB-hRNF14 RBR C417A (178-474) | Expression of human RNF14 RBR domain active site mutant (C417A) protein, codon optimized for E. coli |
| 252506 | pOPINB-yItt1 full-length WT (1-464) | Expression of yeast Itt1 full-length wild-type protein, codon optimized for E. coli |
| 252507 | pOPINB-hRNF14 RBR/yItt1 chimera | Expression of human RNF14 RBR domain (178-453) fused to yeast Itt1 C-terminal tail (452-464), codon optimized for E. coli |
| 252508 | pFUH-hRNF14 full-length WT(1-474) | Lentiviral vector for expressing 3x-FLAG-tagged human RNF14 full-length (1-474) wild-type protein |
| 252509 | pFUH-hRNF14 full-length C417A (1-474) | Lentiviral vector for expressing 3x-FLAG-tagged human RNF14 full-length (1-474) acitve site mutant (C417A) protein |
| 257135 | pFUH-hRNF14/yItt1 chimera | Lentiviral vector for expressing 3x-FLAG-tagged chimera of human RNF14 (residues 1-453) with the C-terminal tail of yeast Itt1 (residues 452-464). |
| 257136 | pOPINB-yItt1/hRNF14 tail chimera | Expression of yeast Itt1 (1-451) fused with human RNF14 C-terminal tail (454-474), codon optimized for E. coli |