pOPINB-yItt1/hRNF14 tail chimera
(Plasmid #257136)

• Purpose: Expression of yeast Itt1 (1-451) fused with human RNF14 C-terminal tail (454-474), codon optimized for E. coli
• Depositing Lab: Bernhard Lechtenberg
• Depositing Organization: Walter and Eliza Hall Institute of Medical Research
  Located in Australia
• Publication: Trevelyan et al 2026 Molecular Cell, 86.

Backbone

• Vector backbone: pOPINB
• Backbone size w/o insert (bp): 5313
• Total vector size (bp): 6735
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: E3 ubiquitin-protein ligase ITT1
• Alt name: Inhibitor of Translation Termination 1
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1353
• Mutation: Deleted C-terminal tail, amino acids 452-464
• GenBank ID: NM_001182427
• Promoter: T7
• Tags / Fusion Proteins:
  • 6xHis-tag (N terminal on backbone)
  • 3C protease cleavage site (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7R

Gene/Insert 2

• Gene/Insert name: RNF14
• Alt name: E3 ubiquitin-protein ligase RNF14
• Alt name: RING finger protein 14
• Alt name: ARA54
• Species: H. sapiens (human)
• Insert Size (bp): 63
• Mutation: C-terminal tail only (454-474)
• GenBank ID: NM_004290
• Entrez Gene: RNF14 (a.k.a. ARA54, HFB30, HRIHFB2038, TRIAD2)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry