pFUH-hRNF14 full-length C417A (1-474)
(Plasmid #252509)

• Purpose: Lentiviral vector for expressing 3x-FLAG-tagged human RNF14 full-length (1-474) acitve site mutant (C417A) protein
• Depositing Lab: Bernhard Lechtenberg
• Depositing Organization: Walter and Eliza Hall Institute of Medical Research
  Located in Australia
• Publication: Trevelyan et al 2026 Molecular Cell, 86.

Backbone

• Vector backbone: pFUH
• Backbone size w/o insert (bp): 10800
• Total vector size (bp): 12220
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RNF14
• Alt name: E3 ubiquitin-protein ligase RNF14
• Alt name: RING finger protein 14
• Alt name: ARA54
• Species: H. sapiens (human)
• Insert Size (bp): 1419
• Mutation: changed Cysteine 417 to Alanine (C417A)
• GenBank ID: NM_004290
• Entrez Gene: RNF14 (a.k.a. ARA54, HFB30, HRIHFB2038, TRIAD2)
• Promoter: UBC
• Tag / Fusion Protein:
  • 3x FLAG-tag (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry