pFUH-hRNF14/yItt1 chimera
(Plasmid #257135)

• Purpose: Lentiviral vector for expressing 3x-FLAG-tagged chimera of human RNF14 (residues 1-453) with the C-terminal tail of yeast Itt1 (residues 452-464).
• Depositing Lab: Bernhard Lechtenberg
• Depositing Organization: Walter and Eliza Hall Institute of Medical Research
  Located in Australia
• Publication: Trevelyan et al 2026 Molecular Cell, 86.

Backbone

• Vector backbone: pFUH
• Backbone size w/o insert (bp): 10800
• Total vector size (bp): 12220
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: RNF14
• Alt name: E3 ubiquitin-protein ligase RNF14
• Alt name: RING finger protein 14
• Alt name: ARA54
• Species: H. sapiens (human)
• Insert Size (bp): 828
• Mutation: tail-less RNF14 (1-453)
• GenBank ID: NM_004290
• Entrez Gene: RNF14 (a.k.a. ARA54, HFB30, HRIHFB2038, TRIAD2)
• Tag / Fusion Protein:
  • 3x FLAG-tag (N terminal on insert)

Gene/Insert 2

• Gene/Insert name: E3 ubiquitin-protein ligase ITT1
• Alt name: Inhibitor of Translation Termination 1
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 42
• Mutation: C-terminal tail only (452-464)
• GenBank ID: NM_001182427

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry