pUNO1-GFP
(Plasmid
#252593)
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PurposeEnhanced green fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in pUNO1 backbone
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252593 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepUNO1
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Backbone manufacturerInvivogen
- Backbone size w/o insert (bp) 3190
- Total vector size (bp) 3910
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Blasticidin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeGFP
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Insert Size (bp)720
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUNO1-GFP was a gift from Jordan Green (Addgene plasmid # 252593 ; http://n2t.net/addgene:252593 ; RRID:Addgene_252593) -
For your References section:
Engineering of episomal plasmid structure to enhance non-viral Poly(beta-amino ester) nanoparticle gene delivery to liver and brain cancer cells. Yang J, Kollings J, Idnani E, Wilson DR, Cozzone IG, Mendes S, Varanasi M, Tzeng SY, Green JJ. PLoS One. 2026 Jul 23;21(7):e0352468. doi: 10.1371/journal.pone.0352468. eCollection 2026. 10.1371/journal.pone.0352468 PubMed 42490567