Engineering of episomal plasmid structure to enhance non-viral Poly(beta-amino ester) nanoparticle gene delivery to liver and brain cancer cells.

Yang J, Kollings J, Idnani E, Wilson DR, Cozzone IG, Mendes S, Varanasi M, Tzeng SY, Green JJ
PLoS One. 2026 Jul 23;21(7):e0352468. doi: 10.1371/journal.pone.0352468. eCollection 2026. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
135046	CAG-eGFP-Z1	Enhanced Green fluorescent protein under constitutive CAG Promoter for mammalian expression in minimal backbone plasmid
135047	CAG-AausFP1-Z1	AausFP1 - bright green fluorescent protein expression from A. australis under CAG promoter in minimal backbone plasmid.
135048	CMV-AausFP1-Z1	AausFP1 - bright green fluorescent protein expression from A. australis under CMV promoter in minimal backbone plasmid.
135049	CMV-eGFP-Z1	Enhanced Green fluorescent protein expression for mammalian cells under constitutive CMV Promoter in minimal backbone plasmid.
135612	EF1α-eGFP-Z1	Enhanced green fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.
135613	EF1α-mNeonGreen-Z1	mNeonGreen monomeric fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.
135614	EF1α-AausFP1-Z1	AausFP1 - bright green fluorescent protein expression from A. australis under EF1a-HTLV promoter in minimal backbone plasmid.
252592	pUNO1-SV40-GFP	Enhanced green fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in pUNO1 backbone with SV40 72bp enhancer
252593	pUNO1-GFP	Enhanced green fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in pUNO1 backbone
252596	pUNO1-fLuc	Firefly luciferase protein expression for mammalian cells under EF1a-HTLV promoter in pUNO1 backbone with SV40 72bp enhancer
252597	pUNO1-mCherry	mCherry protein expression for mammalian cells under EF1a-HTLV promoter in pUNO1 backbone with SV40 72bp enhancer