Leu Cen/ars Tef mNg-MinDec, Tef MinEec-mRuby2
(Plasmid
#252613)
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PurposeCen/ars plasmid for expressing mNg-MinD and MinEec-mRuby2 in budding yeast under Leu2 auxotrophic selection
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252613 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneCen/ars plasmid with Leu2 selection.
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namemNeongreen-MinDec fusion
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SpeciesEscherichia coli
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Tag
/ Fusion Protein
- mNeongreen (N terminal on insert)
Gene/Insert 2
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Gene/Insert nameMinEec-mRuby2
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SpeciesE. coli
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Tag
/ Fusion Protein
- mRuby2 (C terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Leu Cen/ars Tef mNg-MinDec, Tef MinEec-mRuby2 was a gift from Scott Coyle (Addgene plasmid # 252613 ; http://n2t.net/addgene:252613 ; RRID:Addgene_252613) -
For your References section:
Noise-Guided Design of Synthetic Protein Waves in Living Cells. Bolshakov DT, Weix EWZ, Galateo TM, Rajasekaran R, Coyle SM. ACS Synth Biol. 2025 Dec 16. doi: 10.1021/acssynbio.5c00599. 10.1021/acssynbio.5c00599 PubMed 41400440