Noise-Guided Design of Synthetic Protein Waves in Living Cells.
Bolshakov DT, Weix EWZ, Galateo TM, Rajasekaran R, Coyle SM
ACS Synth Biol. 2025 Dec 16. doi: 10.1021/acssynbio.5c00599.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 252605 | 3b-MinDec | MoClo part plasmid expressing E. coli MinD protein as part 3b |
| 252606 | 3a-MinEec | MoClo part plasmid expressing E. coli MinE protein as part 3a |
| 252607 | 3b-MinDpd | MoClo part plasmid expressing P. damselae MinD protein as part 3b |
| 252608 | 3a-MinEpd | MoClo part plasmid expressing P. damselae protein as part 3a |
| 252609 | mNg-MinDec Tef TU | Encodes transcriptional unit with following construction: LS-pTef1-ymNeonGreen-MinDec-tAdh-R1 |
| 252610 | MinEec-mRuby2 Tef TU | Encodes transcriptional unit with following construction: L1-pTef1-MinEec-mRuby-tADH-RE |
| 252611 | Leu Cen/ars Tef mNg-MinDec | Cen/ars plasmid for expressing mNg-MinDec in budding yeast under Leu2 auxotrophic selection |
| 252612 | His Cen/ars Tef MinEec-mRuby2 | Cen/ars plasmid for expressing MinEec-mRuby2 in budding yeast under His3 auxotrophic selection |
| 252613 | Leu Cen/ars Tef mNg-MinDec, Tef MinEec-mRuby2 | Cen/ars plasmid for expressing mNg-MinD and MinEec-mRuby2 in budding yeast under Leu2 auxotrophic selection |
| 252614 | Leu integration Tef mNg-MinDec | Integration plasmid for expressing mNg-MinDec in budding yeast. Locus and selection: Leu2. |
| 252615 | Ura integration Tef MinEec-mRuby2 | Integration plasmid for expressing MinE-mRuby2 in budding yeast. Locus: Ura3; selection: His3 |