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Ura integration Tef MinEec-mRuby2
(Plasmid #252615)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252615 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    Ura3 site integration plasmid with His3 selection marker
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MinEec-mRuby2 fusion
  • Species
    Escherichia coli
  • Tag / Fusion Protein
    • mRuby2 (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Final yeast integration plasmid. Digest with NotI prior to LiOAc-ssDNA transforming in budding yeast.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ura integration Tef MinEec-mRuby2 was a gift from Scott Coyle (Addgene plasmid # 252615 ; http://n2t.net/addgene:252615 ; RRID:Addgene_252615)

  • For your References section:

    Noise-Guided Design of Synthetic Protein Waves in Living Cells. Bolshakov DT, Weix EWZ, Galateo TM, Rajasekaran R, Coyle SM. ACS Synth Biol. 2025 Dec 16. doi: 10.1021/acssynbio.5c00599. 10.1021/acssynbio.5c00599 PubMed 41400440
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