pYO1611
(Plasmid
#252676)
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PurposeProtein expression of 6xHis-TEV-mCerulean HsGABARAP (1-116) in bacteria
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Depositing Lab
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Depositing OrganizationMRC Laboratory of Molecular Biology
Located in United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252676 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepOPTH
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert name6xHis-TEV-mCerulean-HsGABARAP (1-116)
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SpeciesH. sapiens (human)
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Mutation1-116
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Entrez GeneGABARAP (a.k.a. ATG8A, GABARAP-a, MM46)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYO1611 was a gift from Roger Williams (Addgene plasmid # 252676 ; http://n2t.net/addgene:252676 ; RRID:Addgene_252676) -
For your References section:
A GABARAP-PtdIns3K-C1 positive feedback loop at the heart of the phagophore nucleation. Dessus AN, Ohashi Y, Bourguet M, Morgan TE, Nunez A, Manifava M, Ktistakis NT, Williams RL. Nat Commun. 2026 Jul 30;17(1):9473. doi: 10.1038/s41467-026-76135-w. 10.1038/s41467-026-76135-w PubMed 42693081